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T輔助細(xì)胞分化ChIP PCR芯片 T Helper Cell Differentiation ChIP PCR Array

科技服務(wù) > PCR芯片實(shí)驗(yàn)服務(wù) > T輔助細(xì)胞分化ChIP PCR芯片 T Helper Cell Differentiation ChIP PCR Array

T輔助細(xì)胞分化ChIP PCR芯片 T Helper Cell Differentiation ChIP PCR Array

T輔助細(xì)胞分化ChIP PCR芯片T Helper Cell Differentiation ChIP PCR Array
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地區(qū):上海
簡(jiǎn)介:T Helper Cell Differentiation ChIP PCR Array
提供商:SAB
服務(wù)名稱:T Helper Cell Differentiation ChIP PCR Array
T Helper Cell Differentiation ChIP PCR Array

 
ProductSpeciesTechnologyCat. No.
T Helper Cell Differentiation EpiTect ChIP qPCR ArrayHumanHistone ModificationsGH-503A
T Helper Cell Differentiation EpiTect ChIP qPCR ArrayMouseHistone ModificationsGM-503A



The Human T Helper Cell Differentiation EpiTect Chip qPCR Array profiles modified histone and nuclear protein binding to the proximal promoter of a panel of genes regulating the commitment of precursor T cells into specific effector subtypes. The array contains 96 pairs of qPCR primers targeting the 1-kb region downstream of the transcription start sites (TSS) of 84 biological important genes plus 12 appropriate control regions. The genes represented by this array include cytokines, cytokine receptors, transcription factors, and other signaling molecules regulating differentiation into Th1 or Th2 cells as well as specific makers for these subtypes. Profiling the histone modifications and nuclear protein binding events at these gene promoters in your T cell populations will help you correlate these interactions with subtype-specific cellular (Th1) and humoral (Th2) immune responses and immune disorders, such as allergy, asthma, autoimmunity, diabetes, hypersensitivity, and rheumatoid arthritis. The results also provide insights into the epigenetic molecular mechanisms and biological pathways behind T helper cell lineage in your model system. Using Chromatin Immunoprecipitation and this real-time PCR Array, you can easily and reliably analyze the association between your chosen nuclear factors and the promoters for a focused gene panel involved in T helper cell differentiation.




Cytokines & Receptors: CCL5, CCL7, CCR3, CCR4, CCR5, CCR6, IL12B, IL12RB2, IL13, IL13RA1, IL17A, IL17RE, IL18, IL18R1, IL18RAP, IL1R1, IL1R2, IL1RL1, IL2, IL21, IL2RA, IL4, IL4R, IL5, IL9, TNF.
T Helper 1 Subtype Markers: CCR5, HAVCR2, IGSF6, IL12B, IL18, IRF1, SOCS1, SOCS5, TLR4, TLR6, TNF.
T Helper 2 Subtype Markers: CCL5, CCL7, CCR3, CCR4, CEBPB, GFI1, GPR44, ICOS, IL13RA1, IL4R, JAK1, NFATC1, NFATC2.
Transcription Factors: CEBPB, FOSL1, FOXP3, GATA3, GATA4, HOXA10, HOXA3, ID2, IRF4, IRF8, MAF, NFATC1, NFATC2, NR4A1, NR4A3, POU2F2, REL, RELB, RORA, RORC, RUNX1, RUNX3, STAT1, STAT6, TOX, ZBTB7B.
Genes Differentially Expressed and Enriched with H3K4me3 but not with H3K27me3:
Th1 Cells: EOMES, IFNG, IL12RB2, IL18R1, IL18RAP, FASLG, TBX21.
Th2 Cells: ASB2, GATA3, IL13, IL1RL1, IL4, IL5, PPARG.
Th17 Cells: IL17A, IL17RE, IL1R1, IL21, RORA, RORC.
Inducible and Natural T Regulatory (iTreg and nTreg) Cells: CCL4, CCR6, FOSL1, FOXP3, IKZF2, IL9, IRF4, IRF8, MYB, NR4A1, NR4A3, POU2F2, REL, RELB, TGIF1, TNFSF11.
Genes Differentially Methylated at Their Proximal Promoters in Conventional Versus Regulatory T Cells: CACNA1F, CHD7, FOXP3, GATA4, HOPX, HOXA10, HOXA3, ID2, IKZF2, IL1R2, IL2RA, KIF2C, LRRC32, PERP, PKD2, TNFRSF9, TP53INP1, UTS2.


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