細胞表面標志物PCR芯片可用于分析與細胞表面標志物水平有關(guān)的84個關(guān)鍵基因的表達。這些標志物已經(jīng)應(yīng)用于以下研究：1）測定白細胞在癌癥組織中的浸潤程度；2）診斷慢性炎性疾病，如動脈粥樣硬化、哮喘、腸炎和關(guān)節(jié)炎；3）確定T細胞/B細胞系和單克?。?）測定白細胞數(shù)量從而定疾病的階段，發(fā)生、發(fā)展或預(yù)后；5）分析免疫浸潤的原因及其與器官排異反應(yīng)的關(guān)系。通過實時定量PCR的方法，研究者即能夠利用該芯片簡單可靠地同時檢測細胞表面標志物相關(guān)基因的表達。
B-cell Surface Markers: Activated B-cells: CD28, CD38, CD69, CD80, CD83, CD86, DPP4, FCER2, IL2RA, TNFRSF8, CD70 (TNFSF7). Mature B-cells: CD19, CD22, CD24, CD37, CD40, CD72, CD74, CD79A, CD79B, CR2, IL1R2, ITGA2, ITGA3, MS4A1, ST6GAL1. Other B-cell Surface Markers: CD1C, CHST10, HLA-A, HLA-DRA, NT5E. T-cell surface markers: Cytotoxic T-cells: CD8A, CD8B. Helper T-cells: CD4. Activated T-cells: ALCAM, CD2, CD38, CD40LG, CD69, CD83, CD96, CTLA4, DPP4, HLA-DRA, IL12RB1, IL2RA, ITGA1, TNFRSF4, TNFRSF8, CD70 (TNFSF7). Other T-cell Surface Markers: CD160, CD28, CD37, CD3D, CD3G, CD247, CD5, CD6, CD7, FAS, KLRB1, KLRD1, NT5E, ST6GAL1. Natural Killer (NK) Cell Surface Markers: CD2, CD244, CD247, CD7, CD96, CHST10, IL12RB1, KLRB1, KLRC1, KLRD1, NCAM1. Monocyte and Macrophage Cell Surface Markers: Activated Macrophages: CD69, ENG, FCER2, IL2RA. Other Monocyte and Macrophage Surface Markers: C5AR1, CD163, CD40, CD63, CD74, CD86, CHST10, CSF1R, DPP4, FCGR1A, HLA-DRA, ICAM2, IL1R2, ITGA1, ITGA2, S100A8, TNFRSF8, CD70 (TNFSF7). Endothelial Cell Surface Markers: ENG, ICAM2, NOS3, PECAM1, SELP, TEK, VCAM1, VWF. Smooth Muscle Cell Surface Markers: MYH10, MYH9, MYOCD. Dendritic Cell Surface Markers: CD1A, CD209, CD40, CD83, CD86, CR2, FCER2. Mast Cell Surface Markers: C5AR1, FCER1A, FCER2, TPSAB1. Fibroblast (Stromal) Surface Markers: ALCAM, COL1A1, COL1A2. Epithelial Cell Surface Markers: CD1D, KRT18, KRT5, KRT8, EPCAM. Adipocyte Surface Markers: RETN. |
